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Scholar Highlight for Week Ending November 7, 2014

April 30, 2015

Cheryl Triplett

My work day begins with writing down the procedure for the day, set up to run experiments (label test tubes, pull out any reagents needed for the day, mix liquid solutions together that will be needed throughout the day). Then I do my DNA amplification or purification. DNA amplification requires putting a small amount of DNA, and other liquids that help the DNA amplify, in a small capped test tube. The tubes go in a machine that heats up and cools down to provide conditions in which the DNA will replicate. DNA amplification takes about 2 ½ to 3 hours. DNA purification requires Eppendorf tubes (see the picture from my first post) and different reagents at different steps of the procedure. DNA purification also takes about 2-3 hours. After completion of the first test I then  run more tests or experiments. In the case of DNA amplification, the next step is to test to see if the DNA amplified. This process takes a couple of hours. DNA extraction is done throughout the afternoon if you have more samples to extract DNA from.

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